Cardiac Calcification

Rettura, Stamford and Seifter, Paper presented at Northeast Regional Meeting of American Chemical Society (1973),1454 conducted a controlled study of the effect of PHT in mice with predisposition to cardiac calcification. This calcification was enhanced by stress. The authors found that PHT reversed the calcification process as determined grossly, microscopically and by chemical analysis. The authors suggest that this might be one mechanism by which PHT is effective in the prevention of cardiac arrhythmias.

1454. Rettura, G., Stamford, W. and Seifter, E., Reversal of cardiac calcification by diphenylhydantoin, Paper presented at N.E. Regional Meeting of the American Chemical Society, Oct. 1973.

Liao, Seifter, Factor, Yellin and Frater, Journal of Surgical Research (1992),3625 observed that calcification of the myocardium of DBA/2J mice was inhibited or reversed by diets supplemented with phenytoin (100 mg/kg) for 70 days. Calcification of myocardium of right ventricles was noticed in 7 of 12 (58%) of mice receiving a regular diet and in only 3 of 12 (25%) mice reciving a supplement of PHT. The authors also studied the effect of orally administered phenytoin on the calcification of glutaragldehyde-fixed bovine pericardium implanted subcutaneously in three groups (9 each) of growing rats. Group A received regular ground chow for 45 days; Group B received regular ground chow for 90 days; and Group C received regular ground chow for the first 45 days, then 1000 mg PHT was added to each kilogram of basal diet until 90 days postimplantation. Calcium content (g/mg dry weight) of the implants in Group C (given PHT) was significantly less (137 18.6) than Group B (214 34.3) but significantly higher than Group C (79.9 41.5). The tibia calcium content of the PHT group was not significantly different from the 90-day controls (Group B). The authors conclude that orally administered PHT inhibits calcification of glutaraldehyde-fixed bovine pericardial implants preferentially. It does not cause decalcification either of implants that have already calcified or of the bones.

3625. Liao, K., Seifter, E., Factor, S.M., Yellin, E.L., and Frater, R.W., Diphenylhydantoin inhibits calcification of bovine pericardial implants and myocardium a preliminary study, J. Surg. Res., 53(4):349-56, 1992.